p-mapkapk2  (Cell Signaling Technology Inc)

Journal: Biology direct

Article Title: PHF5A regulates the expression of the DOCK5 variant to promote HNSCC progression through p38 MAPK activation.

doi: 10.1186/s13062-023-00396-4

Figure Lengend Snippet: Fig. 7 PHF5A activates the p38 MAPK pathway in HNSCC (A) The expression of PHF5A, p38, p-p38 and the downstream targets p-HSP27, p-MSK1, p-MAPKAPK2, p-ATF2 and p-MEK3 in the p38 MAPK pathway was assessed by Western blot following knockdown and overexpression of PHF5A in HNSCC cells. (B) Colony formation assays showed that p38 MAPK inhibition with SB203580 reversed the pro-colony formation effect of PHF5A in FaDu cells. (C) Cell scratch assays showed that SB203580 reversed the pro-migratory effect of PHF5A in FaDu cells. (D) Transwell invasion assays showed that SB203580 reversed the pro-invasive effect of PHF5A in FaDu cells. NC, negative control; si1, PHF5A siRNA1 group; EV, empty vector group; OE, PHF5A overexpression group

Article Snippet: Primary antibodies against p38, p-p38, p-HSP27, p-MSK1, and p-MAPKAPK2 were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Knockdown, Over Expression, Inhibition, Negative Control, Plasmid Preparation